Our Symposium is undoubtedly distinguishable from similar ones due to its very unique and long-standing tradition (41 organized editions), very high scientific level, international character, the relatively large participation of young scientists, PhD students, and regular students and the flexible approach of the Organizers to the individual preferences and expectations of the participants. Moreover, our goals are to promote interdisciplinary research, to draw the attention of participants to new applications of chromatography and to highlight its universal character. Within the framework of the Symposium, we will offer several thematic sessions, which will be led by respected and internationally recognized chair leaders. During these sessions, we will discuss the different aspects of applications and, in particular, we will try to expose the research activities that are being carried out at the intersection of various fields (chemistry, forensics, Earth sciences, biology, pharmacology, medicine, toxicology, chemometrics, the -omic sciences, etc.). Such a strategy permits highly respected individuals from different professional backgrounds as well as representatives of various fields of science to meet and share ideas in a very inspiring atmosphere. Bearing the on-going analytical issues in mind, including the analysis of the chemical composition of complex samples, we will also draw attention to new research targets as well as new methods and approaches. In collaboration with the leading manufacturers and their local representatives, we will demonstrate the latest technological achievements that are now commercially available in the field of the separation sciences. We will also highlight the importance of all of the stages of the analytical process beginning with collecting samples, storing them and analyzing and interpreting complex and multivariate chromatographic data. Within the program of the Symposium, we have scheduled a unique thematic session that is devoted to chemometrics and its applications in the separation sciences. The methods that are used in chemometrics and its general philosophy support the effective planning and optimization of experiments and facilitate the analysis and interpretation of very complex (multidimensional and multimodal) chromatographic data. During the 42nd edition, we plan to have a discussion panel on new and effective teaching methods in the field of chromatography and related techniques with panelists that represent local and international institutions that have different educational experience as well as designers of the instruments that are for educational purposes. During the first day of the Symposium, we encourage young researchers to attend the workshops, trainings and/or courses that will be offered by leading experts and/or representatives of firms.